Detail Cantuman Kembali
Isolasi Bakteri Bacillus thuringiensis dari Tanah Kota Makassar dan Uji Aktivitas Bioinsektisida terhadap Larva Nyamuk Aedes aegypti
- Bagikan:
An investigation of bacteria
Bacillus thuringiensis
from soil in Makassar have
been done. This study aims to obtain bacterial isol
ates of
Bacillus thuringiensis
as a
potential biological control against diseases cause
d by vector Aedes aegypti.
Bacillus thuringiensis
bacteria isolated from soil by using Ohba and Aiza
wa method.
The medium used are Nutrient Agar (NA) medium and L
uria Bertani 0.25 M acetate
buffer pH 6.8 as selective media. Isolates were the
n characterized and identified based
on colony and cell morphology, Gram staining, spore
staining, and biochemical tests,
these isolates were identified as members of
Bacillus thuringiensis
. Total of 19 colonies
from 3 soil samples were analyzed and 1 colony of
Bacillus thuringiensis
obtained,
which colonies B
6
9 were showing positive results as
Bacillus thuringiensis.
Further testing biopestiside activity using third i
nstar larvae of
Aedes aegypti
were
divided into 6 groups, namely the 5 treatment group
s were given fermented by mixing
the sediment into the water to obtain a concentrati
on of 5%, 10%, 15%, 20%, 25% and 1
control group in the form of distilled water.
The test results showed that these isolates can cau
se mortality ranged from 6,67- 60%
after observation for 24 hours and the highest mort
ality occurred at a concentration of
25% with LC
50
value was 24,62%.
Keywords: Isolation,
Bacillus thuringiensis
, biopesticide,
Aedes aegypti
Noer Fauziah Rahman - Personal Name
Noer Fauziah Rahman, Gemy Nastity Handayany, Mukhri ani - Personal Name
Noer Fauziah Rahman, Gemy Nastity Handayany, Mukhri ani - Personal Name
Far 51
Far 51
CD-ROM
Indonesia
perpustakaan FKIK
2014
Farmasi
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APA Citation
Noer Fauziah Rahman. (2014).Isolasi Bakteri
Bacillus thuringiensis
dari Tanah Kota Makassar dan
Uji Aktivitas Bioinsektisida terhadap Larva Nyamuk
Aedes aegypti.(Electronic Thesis or Dissertation). Retrieved from https://localhost/etd