Noer Fauziah Rahman Primary Author Noer Fauziah Rahman, Gemy Nastity Handayany, Mukhri ani Primary Author mixed material bibliography Farmasi perpustakaan FKIK 2014 monographic id Indonesia

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An investigation of bacteria Bacillus thuringiensis from soil in Makassar have been done. This study aims to obtain bacterial isol ates of Bacillus thuringiensis as a potential biological control against diseases cause d by vector Aedes aegypti. Bacillus thuringiensis bacteria isolated from soil by using Ohba and Aiza wa method. The medium used are Nutrient Agar (NA) medium and L uria Bertani 0.25 M acetate buffer pH 6.8 as selective media. Isolates were the n characterized and identified based on colony and cell morphology, Gram staining, spore staining, and biochemical tests, these isolates were identified as members of Bacillus thuringiensis . Total of 19 colonies from 3 soil samples were analyzed and 1 colony of Bacillus thuringiensis obtained, which colonies B 6 9 were showing positive results as Bacillus thuringiensis. Further testing biopestiside activity using third i nstar larvae of Aedes aegypti were divided into 6 groups, namely the 5 treatment group s were given fermented by mixing the sediment into the water to obtain a concentrati on of 5%, 10%, 15%, 20%, 25% and 1 control group in the form of distilled water. The test results showed that these isolates can cau se mortality ranged from 6,67- 60% after observation for 24 hours and the highest mort ality occurred at a concentration of 25% with LC 50 value was 24,62%. Keywords: Isolation, Bacillus thuringiensis , biopesticide, Aedes aegypti Far 51 Repositori FKIK UINAM Perpustakaan FKIK UIN Alauddin Makassar Far 51 Far 51 My Library (Multimedia FKIK) Far 51 Far 51` My Library (Multimedia FKIK) Far 51 Isolasi Bakteri Bacillus thuringiensis dari Tanah Kota Makassar dan Uji Aktivitas Bioinsektisida terhadap Larva Nyamuk Aedes aegypti Isolasi Bakteri Bacillus thuringiensis dari Tanah Kota Makassar dan Uji Aktivitas Bioinsektisida terhadap Larva Nyamuk Aedes aegypti SAMPUL_SKRIPSI_IMPUT.jpg.jpg 296 2020-03-11 09:06:28 2020-03-11 09:09:10 machine generated